anti prostaglandin endoperoxide synthase 2 (Proteintech)

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Proteintech anti prostaglandin endoperoxide synthase 2

PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and <t>PTGS2</t> in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.

Anti Prostaglandin Endoperoxide Synthase 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2+ptgs2/COX2%2F+Cyclooxygenase+2%2F+PTGS2+Antibody/pmc13040322-90-57-66
Average 96 stars, based on 1092 article reviews

anti prostaglandin endoperoxide synthase 2 - by Bioz Stars, 2026-10

96/100 stars

Images

1) Product Images from "Peroxiredoxin 4 suppresses ferroptosis in esophageal squamous cell carcinoma by activating the phosphoinositide 3-kinase signaling pathway"

Article Title: Peroxiredoxin 4 suppresses ferroptosis in esophageal squamous cell carcinoma by activating the phosphoinositide 3-kinase signaling pathway

Journal: Biomedical Reports

doi: 10.3892/br.2026.2133

Figure Legend Snippet: PRDX4 is an important regulator of ferroptosis in ESCC cells. (A) Determination of MDA, LPO and GSH contents in KYSE270 cells after transfection with PRDX4 siRNA. (B) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (C) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE270 cells transfected with PRDX4 siRNA. (D) Determination of MDA, LPO and GSH contents in KYSE30 cells after transfection with pcDNA3.1-PRDX4. (E) Western blot analysis of the protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (F) The relative protein levels of GPX4, SLC7A11 and PTGS2 in KYSE30 cells transfected with pcDNA3.1-PRDX4. (G) Detection of the levels of MDA, LPO and GSH in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (H) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (I) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the control group, PRDX4 siRNA group and PRDX4 siRNA plus Fer-1 group in KYSE270 cells. (J) Detection of the levels of MDA, LPO and GSH in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (K) Western blot analysis of the protein expression levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. (L) The relative protein levels of GPX4, SLC7A11 and PTGS2 in the pcDNA3.1 group, pcDNA3.1-PRDX4 group and pcDNA3.1-PRDX4 plus erastin group in KYSE30 cells. ** P<0.01, *** P<0.001 and **** P<0.0001, indicate statistical significance. PRDX4, peroxiredoxin 4; ESCC, esophageal squamous cell carcinoma; siRNA, small interfering RNA; MDA, malondialdehyde; LPO, lipid peroxidation; GSH, glutathione; GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; PTGS2, prostaglandin-endoperoxide synthase 2; Fer-1, ferrostatin-1; ns, not significant.

Techniques Used: Transfection, Western Blot, Control, Expressing, Small Interfering RNA

other:

Article Title: Apoptotic Bodies Derived from Fibroblast-Like Cells in Subcutaneous Connective Tissue Inhibit Ferroptosis in Ischaemic Flaps via the miR-339-5p/KEAP1/Nrf2 Axis.
Article Snippet: Preventing and treating avascular necrosis at the distal end of the flaps are critical to surgery success, but current treatments are not ideal.. A recent study shows that apoptotic bodies (ABs) generated near the site of apoptosis can be taken up and promote cell proliferation.. The study reveals that ABs derived from fibroblast-like cells in the subcutaneous connective tissue (FSCT cells) of skin flaps promoted ischaemic flap survival.

Article Title: Apoptotic Bodies Derived from Fibroblast‐Like Cells in Subcutaneous Connective Tissue Inhibit Ferroptosis in Ischaemic Flaps via the miR‐339‐5p/KEAP1/Nrf2 Axis
Article Snippet: Antibodies against KEAP1 (cat# 10503‐2‐AP), SLC7A11/xCT (cat# 26864‐1‐AP), TXNRD1 (cat# 11117‐1‐AP), GPX4 (cat# 67763‐1‐Ig), NOX1 (cat# 17772‐1‐AP), COX2/cyclooxygenase 2/PTGS2 (COX2, cat# 12375‐1‐AP), AGO2 (cat# 67934‐1‐Ig) and histone H3 (cat# 17168‐1‐AP) were obtained from the Protein Technology Group (Chicago, IL, USA).

Incubation:

Article Title: Microgel-based carriers enhance skeletal stem cell reprogramming towards immunomodulatory phenotype in osteoarthritic therapy
Article Snippet: .. The membranes were incubated with anti-vinculin (proteintech, 1:5000), anti-phospho-YAP (CST, 1:2000), anti-YAP (CST, 1:2000), anti-COX2/Cyclooxygenase 2/Ptgs2 (proteintech, 1:1000), anti-Lamin b1 (proteintech, 1:2000) and anti-GAPDH (CST, 1:2000) at 4 °C for 8–12 h. After that, the membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Servicebio, 1:3000) or HRP-conjugated goat anti-mouse IgG (Servicebio, 1:3000) at room temperature for 1 h. .. The samples were washed with PBS, fixed with 4 % paraformaldehyde for 30 min, according to experimental requirements, were incubated with anti-Pdpn (proteintech, 1:200), anti-Grem1 (CST, 1:50), anti-Cd200 (proteintech, 1:200), anti-Ctsk (Abcam, 1:200), anti-Ptgs2 (proteintech, 1:200), anti-vinculin (proteintech, 1:200) or anti-YAP (CST, 1:200) at 4 °C for 8–12 h. After that, samples incubated with goat anti-rabbit IgG (Servicebio, Alexa Fluor 488, 1:300), goat anti-rabbit IgG (Servicebio, Cy3, 1:300) or goat anti-mouse IgG (Servicebio, Alexa Fluor 488, 1:200) for 1 h at room temperature.

Recombinant:

Article Title: Serotype-specific host proteome remodeling in human foreskin fibroblasts during lytic HSV-1 and HSV-2 infection
Article Snippet: Membranes were blocked with 5% skimmed milk for at least 2 h, followed by incubation overnight at 4 °C with primary antibodies diluted in antibody dilution buffer (Beyotime, P0256-500 mL). .. The following primary antibodies were used: PIBF1 (Polyclonal, Proteintech, 14,413–1-AP, 1:5000), TFPI2 (Recombinant, Proteintech, 83,279–1-RR, 1:5000), KIF22 (Polyclonal, Proteintech, 13,403–1-AP, 1:2000), Cystatin C (Recombinant, Proteintech, 82,441–1-RR, 1:5000), PLXNB1 (Abmart, PK35830, 1:1000), COX2/Cyclooxygenase 2/PTGS2 (Polyclonal, Proteintech, 12,375–1-AP, 1:4000), Podocalyxin (Polyclonal, Proteintech, 18,150–1-AP, 1:1000), MID1 (Abmart, PA7018S, 1:4000), ICP5 (Abcam, ab6508, 1:1000), β-actin (Proteintech, 66,009–1-Ig, 1:20,000). ..

Article Title: Serotype-specific host proteome remodeling in human foreskin fibroblasts during lytic HSV-1 and HSV-2 infection.
Article Snippet: Membranes were blocked with 5% skimmed milk for at least 2 h, followed by incubation overnight at 4 °C with primary antibodies diluted in antibody dilution buffer (Beyotime, P0256-500 mL). .. The following primary antibodies were used: PIBF1 (Polyclonal, Proteintech, 14,413–1-AP, 1:5000), TFPI2 (Recombinant, Proteintech, 83,279–1-RR, 1:5000), KIF22 (Polyclonal, Proteintech, 13,403–1-AP, 1:2000), Cystatin C (Recombinant, Proteintech, 82,441–1-RR, 1:5000), PLXNB1 (Abmart, PK35830, 1:1000), COX2/Cyclooxygenase 2/PTGS2 (Polyclonal, Proteintech, 12,375–1-AP, 1:4000), Podocalyxin (Polyclonal, Proteintech, 18,150–1- AP, 1:1000), MID1 (Abmart, PA7018S, 1:4000), ICP5 (Abcam, ab6508, 1:1000), β-actin (Proteintech, 66,009– 1-Ig, 1:20,000). ..

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